IVF / ICSI- Test Tube Baby Treatment

IVF / ICSI

What is IVF ?

IVF stands for In-vitro fertilization. It refers to the process by which a woman’s eggs are collected and then fertilized with sperms outside her womb in the laboratory.

Washed sperms are added in the petridish where eggs are collected so that one of the sperm fertilizes the egg.

The treated eggs (undeveloped organisms) are then refined in the research center and after suitable development; they are moved back to the uterine depression.

It thus bypasses the fallopian tubes completely.

What is ISCI ?

ISCI represents Intra Cytoplasmic Sperm Injection in which sperm is infused into the oocyte as opposed to putting them together and permitting them to normally prepare. ICSI is hence an extremely powerful strategy to get preparation of eggs

It is finished via prepared embryologist utilizing particular miniature control devices and hardware and altered magnifying instruments which assist them with choosing the most morphologically ordinary and dynamic sperm to be infused into the oocyte cytoplasm.

This usually results in normal fertilization in approximately 70-85% of eggs injected with viable sperm.

What are the Indications for IVF ?

  • Women with tubal diseases like blocked tubes, hydrosalpinx, and previous tubectomy done
  • Patent tube however non-practical like in past pelvic provocative sickness or tubal tuberculosis
  • Infertility due to endometriosis
  • Unexplained infertility
  • Infertility because of male variables like oligospermia (low count), asthenozoospermia (less motility), teratozoospermia (unusual sperms) or a mix of anomalies like oligoasthenoteratozoospermia

What are indications of ICSI ?

  • Recently bombed IVF cycle because of bombed treatment
  • Severe male factor Infertility- Oligoasthenoteratozoopermia- that is very low count, low motility or high percentage of abnormal sperms
  • Sperms got through TESA (Trans Epididymal Sperm Aspiration), PESA (Percutaneous Epididymal Sperm Aspiration ), TESE (Trans Epididymal Sperm Aspiration) or Testicular Biopsy-sperms are gotten microsurgically from testis or epididymis

The purpose of IVF/ICSI treatment is to

  • By pass the fallopian tube where treatment typically happens, with the goal that tubal element is overcomed
  • By eliminating the oocyte, the nature of egg can be evaluated
  • Unobtrusive hormonal deformities get revised and endometrial receptivity moves along
  • ICSI sidesteps intrinsic shortcoming of preparation of ordinary looking sperm like acrosome defet and so forth.

What is TESA /TESE /PESA ?

In men with obstructive azoospermia (on account of conduit blockage or nonattendance of the vas deferens), sperm are generally recuperated from the epididymis by penetrating it with a fine needle. This technique is called PESA (percutaneous epididymal sperm goal).

In TESA or testicular sperm desire (otherwise called TESE, or testicular sperm extraction), testicular tissue is sucked out through a fine needle, under neighborhood sedation. The sperm are then freed from tissue and utilized for ICSI.

What is the success rate of IVF Treatment / ICSI ?

Normal achievement rate is around 30-35% yet changes from one case to another. Women with great ovarian hold and PCOS have better cummulative achievement rates (60-70%) though women with poor ovarian save, harmed endometrium have unfortunate achievement rate (20-30%).

  • Success rate depends on
  • Age of couple
  • Pre IVF assessment utilization of OC pills, Use of estradiol valerate, utilization of leuprolide
  • Stimulation protocol used
  • Nature of gametes (eggs and sperms) & incipient organisms
  • Number of embryos transferred

Procedure of IVF ?

  • It starts on Day2 of menses. Baseline ultrasonography is done to lead of ovarian growths. Whenever required, pattern chemicals like Serum E2, LH and P4 might be done to affirm that the ovaries are smothered
  • On premise of Age, BMI, Previous excitement result, AFC and AMH , Injections are begun
  • Development of follicle and endometrium is checked by sequential ultrasonography . Blood tests if required are done
  • Portion of infusions is expanded, diminished or kept up with, infusions are added as and when required
  • Following 9 to 10 days of Injections , when at least 2 follicles are more than 18mm in measurement, trigger infusion is arranged
  • 35 hours post trigger, oocyte get is done.Oocyte recovery is a surgery which is done under sedation where the follicles are aspirated
  • Aspirated follicles will be looked for oocytes, or eggs. Only one out of every odd follicle will contain an oocyte
  • Every one of the oocytes which are recovered are painstakingly distinguished and kept in the CO2 hatchery
  • Semen test is gotten and semen handling done which segregates the best sperms
  • IVF/ICSI is performed in laboratory
  • Arrangement of undeveloped organisms and development of undeveloped organisms are observed in the lab
  • 3 to 5 days post fertilzation, undeveloped organism move is finished. Incipient organism move is a straightforward method very much like an IUI technique done under sonography direction. At the point when the incipient organisms are moved on day 3, the incipient organism is supposed to be 8 cell stage. At the point when the undeveloped organisms are moved on day 5, it is at the blastocyst stage. This is the most elevated type of human existence that can be supported in the research center
  • Assuming there are surplus great undeveloped organisms , one can freeze them
  • Luteal support is given
  • Patient is approached to trail not very far behind 15 days for result

More about IVF ?

  • Treatment cycle can be dropped on the off chance that enough follicles don't develop. It as a rule occurs in more seasoned ladies and unfortunate responders. It occurs in around 5-6% of IVF treatment cycles.In such cases, various meds or conventions can be utilized in the following cycle
  • Now and again, inspite of follicles being shaped, egg may not be there..that is vacant follicle. It happens because of ill-advised trigger, incapable trigger. It might require change in convention and treatment routine
  • Undeveloped organism move might be delayed and all incipient organisms frozen assuming there are extreme follicles that structure like in PCOS
  • There is an expanded opportunity of different pregnancies and ectopic pregnancies because of the exchange of numerous incipient organisms. However, the risks for pregnancy i.e. confusions essentially during pregnancy because of IVF treatment/ICSI & it are unaltered to during conveyance
  • Risk of birth abandons after ICSI is equivalent to for children considered through IVF treatment without ICSI, and for those imagined normally. Be that as it may, in extreme male component fruitlessness related with adjusted movements and Y chromosome miniature cancellation, transmission might happen to the male posterity
  • It doesn't need bed rest or delayed leave. It doesn't need over night confirmation
  • Associated Endometriosis might influence the egg quality and implantaion
  • Associated Fibroids, contingent upon size and area might require treatment
  • Associated Hydrosalphix ought to be dealt with, as the fliud influences implantation and is embryotoxic
  • Practice and solid eating routine assistance to battle oxidant stress and further develop result
  • Life pattern of sperm is around 90 days, consequently the sperms that are removed are created 3 months prior.Hence utilization of liquor, cigarette, tobacco ought to be kept away from. Continuous discharge (each 2 to 3 days) ought to be suggest
  • Now and again, pre cycle Hysteroscopy might be suggested

What is Natural cycle IVF ?

  • In women with poor ovarian save, giving exorbitant infusions doesn't work on the result concerning amount and nature of eggs. It rather adds to cost and injury to the patient
  • Natural cycle IVF utilizes negligible or no medications to enroll normally chosen follicle. It is very practical and gives an opportunity of origination to women with poor ovarian reserve
  • Here instead of zeroing in on the amount of the eggs the attention is on the nature of the egg gathered
  • The uterine coating is more responsive as there is insignificant utilization of drug prompting better implantation
  • Eggs can likewise be pooled utilizing different cycles and afterward moved back to the uterus
  • The achievement pace of Natural Cycle IVF is dependent on a different variables that are past the control of the specialists. In this manner the achievement paces of Natural Cycle IVF are similarly low when contrasted with Stimulated Cycle IVF fertility treatment & IVF Clinic In Thane